Proteomics Submission Guidelines
Date modified 06/15/07.
Please, read the ESI LC MS/MS sample analysis policy.
To reserve your place in queue, please send e-mail to pechan@ra.msstate.edu (please no phone calls) with this information:
- Number of samples.
- Is "Spot Cutting" and Protein Digestion at the LSBI requested?
- Mass Spectrometry service requested:
- ESI 1-D LC or 2-D LC (ProteomeX LCQ, ThermoFinnigan)
- MALDI TOFTOF simple sample; minimum 50 samples required (ABI 4700, Applied Biosystems)
- Contact name and phone number.
- PI Name.
Do so only when your samples are physically existent and you know the exact
number of samples.
When your samples advance in the queue, you will be contacted and asked to
bring them to LSBI. Principal investigators can view the
queue here.
When preparing samples for LC MSMS analysis, last steps must include this
simple
procedure.
For robotic in-gel protein digestion followed by MALDI MSMS analysis, bring
to LSBI freshly made solutions according to this
list.
At the moment of sample submission, the following form must be completed:
LSBI Mass Spec
Sample Submission Form
iTRAQ Nano 2D LC Sample Submission Form
Please review the following tables showing the standard 2-D LC methods
(gradients) LSBI offers.
Long
Reverse Phase Methods (LRP)
10 Steps
Method (10ST)
20 Steps
Method (20ST)
Databases kept and regularly updated at LSBI:
Human
Mouse
Chicken
Arabidopsis
Corn
Rice
NCBI Nr
The resource for the databases above is the National Center for
Biotechnology Information (http://www.ncbi.nih.gov/).