Imaging & Quantification (Nucleic Acids)
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Alpha Innotech AlphaScanThe AlphaScan has 2 lasers that image Cy3- and Cy5-labeled microarray, tissue array, and cell array slides. Scanning resolution ranges from 40 um to 5 um. A broad adjustable focus range (+ 250 um), adjustable laser power and PMT settings provide maximum flexibility for different substrates and signal intensity. LSBI offers three software packages for data analysis. For spot finding and data extraction, we provide AlphaScan Analysis Software and Axon GenePix Pro Software (v.6.0). For normalization, statistical analysis, clustering analysis, and data visualization, the LSBI has Axon Acuity Software (v.4.0). AlphaScan User Manual | Request AlphaScan Analysis Software - 4 dongles available LSBI Contact: Amanda Cooksey |
GE HealthCare Typhoon 9410 WorkstationThe Typhoon 9410 is the state-of-the-art gel and blot imager with the capability of microarray analysis. It can detect three types of chemistries: storage phosphor, fluorescence and chemiluminescence. LSBI Contact: Dr. Tibor Pechan |
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Bio-Rad VersaDoc 3000The VersaDoc 3000 is a multi-imager for visualizing non-isotopic fluorescent, chromogenic and chemiluminescent-labeled samples. The VersaDoc has UV and white light transmissive illumination for image capturing and analysis of blots, film and opaque samples. LSBI Contact: Amanda Cooksey |
Agilent 2100 BioanalyzerThe 2100 Bioanalyzer is a microfluidics-based Lab-on-a-Chip for the analysis of RNA and proteins. The Bioanalyzer provides accurate sizing and quantitation information and takes about 45 to 30 minutes to run each set of 10 to 12 samples, depending upon whether you are running protein or RNA, respectively. Currently, we have the following LabChip kits in stock: the RNA 6000 Pico kit (200 - 5000 pg/ul total RNA), the RNA 6000 Nano kit (25-500 ng/ul total RNA), and the Protein 230 kit (14 - 230 kDa).
Expert
User's Guide |
Troubleshooting Guide LSBI Contact: Amanda Cooksey |
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Nanodrop ND-1000 SpectrophotometerThe Nanodrop ND-1000 is a full-spectrum UV/Vis spectrophotometer that can be used to check concentration of nucleic acids, proteins, and for certain fluorescent dyes, labeling efficiency of hybridization probes and protein conjugates. It requires 1 to 2 ul of sample and takes only seconds to read. There is currently no charge to use the Nanodrop. LSBI Contact: Amanda Cooksey |
Molecular Devices Spectramax Gemini XSTunable Microplate Spectrofluorometer for Fluorescence, Time-Resolved Fluorescence and UV/VIS Readings: The Spectrofluorometer is the instrument of choice for the measurement of low level detection limits of fluorescence based assays used for cellular and molecular binding studies, DNA quantitation, drug screening and environmental analysis. LSBI Contact: Ken Pendarvis |
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Molecular Devices Spectramax Plus 384UV/Vis Microplate/Cuvette Spectrophotometer: The Spectromax Plus is capable of UV/Vis spectrum of 190-1000 nm, tunable in 1 nm increments using either microplates or cuvettes. This instrument can be used for colorimetric assays, ELISAs/EIAs, immunoassays, drug dissolution profiles, enzyme kinetics, etc. LSBI Contact: Ken Pendarvis |
Molecular Devices Lmax Microplate LuminometerLuminescence Microplate reader: The Lmax is a microplate luminometer designed for the measurement of chemi- and bioluminescence. This instrument will allow such applications as assays for single and dual luciferase reporter genes, ATP, dsDNA and luminescence-based ELISA. LSBI Contact: Ken Pendarvis |
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