Affymetrix GeneChip^® Expression Analysis

The Affymetrix GCS3000 is a veratile instrument that can be used for gene expression analysis (GeneChip^® Expression Arrays), large-scale genotyping experiments (GeneChip^® Mapping Arrays), and for rapid resequencing of entire genomes or portions therof.

Affymetrix Array Price List | Getting Started

Gene Expression Studies: GeneChip^® Expression Analysis Technical Manual
Protocols for RNA target preparation are mandatory reading for Affy Users.
Presentation: Principles of  Expression Analysis, Experimental Design, Statistical Analysis, and Biological Interpretation
Data Analysis Fundamentals Manual
GeneChip Whole Target (WT) Sense Target Labeling Assay Manual v4

DNA Data Analysis Studies (Mapping, Resequencing):
Presentation: DNA Data Analysis Workshop
GeneChip^® Mapping Assay Manual
CustomSeq Custom Resequencing Array Design Guide
CustomSeq Resequencing Array Protocol

LSBI Contact: Amanda Cooksey

Alpha Innotech AlphaScan

The AlphaScan has 2 lasers that image Cy3- and Cy5-labeled microarray, tissue array, and cell array slides. Scanning resolution ranges from 40 um to 5 um. A broad adjustable focus range (+ 250 um), adjustable laser power and PMT settings provide maximum flexibility for different substrates and signal intensity. LSBI offers three software packages for data analysis. For spot finding and data extraction, we provide AlphaScan Analysis Software and Axon GenePix Pro Software (v.6.0). For normalization, statistical analysis, clustering analysis, and data visualization, the LSBI has Axon Acuity Software (v.4.0).

AlphaScan User Manual | Request AlphaScan Analysis Software - 4 dongles available

LSBI Contact: Amanda Cooksey